数据质控报告
1 测序reads统计
测序reads统计如下表：
表1 测序数据统计
| Type | Values |
|---|---|
| Number of Reads | 399967594 |
| Valid Barcodes | 339047468 |
| Valid UMIs | 395738572 |
注：
Number of Reads：reads总数；
Valid Barcodes：包含有效Barcode的reads数 ；
Valid UMIs：包含有效UMIs的reads数。
2 数据比对
比对结果统计如下表：
表2 比对结果统计
| Type | Values |
|---|---|
| Reads Mapped to Genome | 96.01% |
| Reads Mapped Confidently to Genome | 80.77% |
| Reads Mapped Confidently to Intergenic Regions | 1.69% |
| Reads Mapped Confidently to Intronic Regions | 15.62% |
| Reads Mapped Confidently to Exonic Regions | 63.44% |
| Reads Mapped Confidently to Transcriptome | 71.64% |
注：
Reads Mapped to Genomes：比对到参考基因组上的Reads在总Reads中占的比例；
Reads Mapped Confidently to Genome：比对到参考基因组并得到转录本GTF信息支持的Reads在总Reads中占的比例；
Reads Mapped Confidently to Intergenic Regions：比对到基因间区域的Reads在总Reads中占的比例；
Reads Mapped Confidently to Intronic Regions：比对到内含子区域的Reads在总Reads中占的比例；
Reads Mapped Confidently to Exonic Regions：比对到外显子区域的Reads在总Reads中占的比例；
Reads Mapped Confidently to Transcriptome：比对到已知参考转录本的Reads在总Reads中占的比例。
3 分析统计
Cell分析统计如下：
表3 Cell统计
| Type | Values |
| Estimated Number of Cells | 7104 |
| Median UMI Counts per Cell | 7261.0 |
| Median Genes per Cell | 2624.0 |
| Total Genes Detected | 37416 |
| Sequencing Saturation | 73.22% |
| Fraction Reads in Cells | 77.39% |
注：
Estimated Number of Cells：检测到的细胞数；
Median UMI Counts per Cell：每个cell的UMI中位数；
Median Genes per Cell：每个cell中基因的中位数；
Total Genes Detected：基因总数；
Sequencing Saturation：测序饱和度；
Fraction Reads in Cells：过滤后细胞reads数占总reads数比例。
图2 Barcode rank统计图
